oa Undifferentiated iPS Cells Do Not Regenerate Functional Lung Tissue When Seeded on Native Lung Extracellular Matrix under Biomimetic Culture Conditions

Perfusion-decellularized native lungs seeded with human BJ

RNA-induced pluripotent stem (BJ-RiPS) and umbilical vein endothelial cells failed to regenerate functional lung tissue as quantified by immunohistochemistry (no detection of TTF1,CC10,and Pro-SPB), gene expression (non-significant differences in lung-specific gene expression as compared to cells cultured under standard conditions), and in vitro lung gas exchange properties. Histological analysis of orthotopically transplanted BJ-RiPS lungs revealed a teratoma (detection of ectoderm: TuJ1, mesoderm: SMA, and endoderm: TTF1).