1887
Volume 2 (2021) Number 1
  • EISSN: 2708-0463

Abstract

تستخدم هذه الدراسة الماء المقطر لاستخلاص صبغة الأنثوسيانين من بذور الرمان وأوراق اللهانة البنفسجية، وذلك أن الماء ليس له أي تأثير سُمّي، ويتبخر عند درجات الحرارة المنخفضة من دون التأثير في التركيب الكيمياوي للصبغة المستخلصة. وتبين الدراسة أن الصبغة الحمراء المستخلصة من الرمان كانت ذات وسط حامضي (pH= 3.0)، بينما كانت الصبغة البنفسجية المستخلصة من اللهانة ذات وسط حامضي خفيف (pH=6.0)، وبلغت تراكيز الصبغة في عصير كلا النباتين بعد إسقاط القيمة الامتصاصية لكل منها على منحنى المحاليل القياسية 130 و 503 ملغم/ 100 مل من عصير كل نبات، على التعاقب، ونجحت هذه الصبغة، بلونيها الأحمر والبنفسجي، في صبغ مسحات البكتيريا والمقاطع النسيجية الحيوانية والنباتية، وباستخدام مثبّتي اليود وكبريتات النحاس، على التعاقب، وعلى نحو يضاهي الصبغات الكيمياوية المتعارف عليها. كما كان لهذه الصبغة تأثير فعال في صبغ الأقمشة القطنية والصوفية على نحو جيد باستخدام عصير الليمون وصودا الخبز كمثبّت.

In this study we used distilled water to extract pigment anthocyanin from seeds of the pomegranate and violet cabbage leaves because the water does not have any toxic effect, their evaporates happened at low temperatures, and without influence on the chemical composition of the extracted dye. It was found that the red dye extracted from the pomegranate had an acidic medium (pH 3.0), while the violet dye extracted from the cabbage leaves had a light acid medium (pH 6). The amount of dye in the juice of both plants after dropping the absorbance value of each on the curve of standard solutions was 310 and 50 0mg/ 100 ml of juice from each plant, respectively. This dye, with its red and violet colors, succeeded in dyeing bacterial smears, animal, and plant tissue sections, with iodine and copper sulfate fixers respectively, and in a way similar compared to conventional chemical dyes. It was also an effective dye in the dyed cotton and wool fabrics well with lemon juice and baking soda as add fixative.

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2021-04-30
2024-11-08
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References

  1. Jackman RL, Smith JL. Anthocyanins and betalains. In: Hendry GAF, Houghton JD, editors. Natural food colorants. 2nd ed. London: Blackie; 1996. pp. 244–309.
  2. Vukosavljević P, Bukvić B, Janković M, Mašović S. Change of anthocyanins content during raspberry extraction. Journal of Agricultural Sciences. 2003;48(1):85–102.
  3. Chigurupati N, Saiki L, Gayser C, Dash AK. Evaluation of red cabbage dye as a potential natural color for pharmaceutical use. International Journal of Pharmaceutics. 2002;241(2):293–299.
  4. Lebling RW, Pepperdine D. Natural remedies of Arabia. Saudi Aramco World. 2006;57(5):12–21.
  5. Zhou H, Wu L, Gao Y, Ma T. Dye-sensitized solar cells using 20 natural dyes as sensitizers. Journal of Photochemistry and Photobiology A: Chemistry. 2011;219(2–3):188–194.
  6. Clifford MN. Anthocyanins – Nature, occurrence and dietary burden. Journal of the Science of Food and Agriculture. 2000;80(7):1063–1072.
  7. Kong JM, Chia LS, Goh NK, Chia TF, Brouillard R. Analysis and biological activities of anthocyanins. Phytochemistry. 2003;64(5):923–933.
  8. Rossi A, Serraino I, Dugo P, Di Paola R, Mondello L, Genovese T, et al. Protective effects of anthocyanins from blackberry in a rat model of acute lung inflammation. Free Radical Research. 2003;37(8):891–900.
  9. Harbome JB, Grayer RJ. The anthocyanins. In: Harborne JB, editor. The flavonoids. London: Chapman and Hall; 1988. pp. 1–20.
  10. Bancroft JD, Gamble M. Theory and practice of histological techniques. New York: Churchill Livingstone;4thEd.,2007.pp.744.
  11. Dangles O, Saito N, Brouillard R. Kinetic and thermodynamic control of flavylium hydration in the pelargonidin-cinnamic acid complexation. Origin of the extraordinary flower color diversity of Pharbitis nil. Journal of the American Chemical Society. 1993;115(8):3125–3132.
  12. Kallio H, Pallasaho S, Kärppä J, Linko RR. Comparison of the half-lives of the anthocyanins in the juice of crowberry, Empetrum nigrum. Journal of Food Science. 1986;51(2):408–410.
  13. محمد، سعيد حميد. التأثيرات الحيوية للأنثوسيانيات المستخلصة من ثمرة البرتقال في بعض أنواع البكتيريا. مجلة ابن الهيثم للعلوم الصرفة والتطبيقية. 2014؛27(3):110-117.
  14. المضافات الغذائية: صبغة الأنثوسيانين. مجلة اخبار الخليج (البحرين، 1437هـ)، العدد 13934.
  15. Zhu F, Yuan Z, Zhao X, Yin Y, Feng L. Composition and contents of anthocyanins in different pomegranate cultivars. Acta Horticulturae. 2015;1089:35–41.
  16. Silva S, Costa EM, Calhau C, Morais RM, Pintado ME. Anthocyanin extraction from plant tissues: A review. Critical Reviews in Food Science and Nutrition. 2017;57(14):3072–3083.
  17. Liu X, Xiao G, Chen W, Xu Y, Wu J. Quantification and purification of mulberry anthocyanins with macroporous resins. Journal of Biomedicine and Biotechnology. 2004;2004(5):326–331.
  18. Lee J, Rennaker C, Wrolstad RE. Correlation of two anthocyanin quantification methods: HPLC and spectrophotometric methods. Food Chemistry. 2008;110(3):782–786.
  19. Sondheimer E, Kertesz ZI. The anthocyanin of strawberries. Journal of the American Chemical Society. 1948;70(10):3476–3479.
  20. Black J.G. Microbiology: principles and applications. Englewood Cliffs, NJ: Prentice Hall; 1993.
  21. Al-Hajj HA. Light microscopic techniques: Theory and practice. Amman, Jordan: Jordan Book Center; 1998.
  22. Salam MA, Salam A. Study on color fastness properties onto bleached sulfonated jute–cotton blended fabrics with basic dyes. Journal of Textile and Apparel, Technology and Management. 2005;4(4):1–6.
  23. Adeel S, Ali S, Bhatti, IA, Zsila F. Dyeing of cotton fabric using pomegranate (Punica granatum) aqueous extract. Asian Journal of Chemistry. 2009;21(5):3493–3499.
  24. Ncube NS, Afolayan AJ, Okoh AI. Assessment techniques of antimicrobial properties of natural compounds of plant origin: Current methods and future trends. African Journal of Biotechnology. 2008;7(12):1797–1806.
  25. Mortazavi SM, Kamali Moghaddam M, Safi S, Salehi R. Saffron petals, a by-product for dyeing of wool fibers. Progress in Color, Colorants and Coatings. 2012;5(2):75–84.
  26. Brouillard R. Chemical structure of anthocyanins. In: Markakis P, editor. Anthocyanins as food colors. New York: Academic Press; 1982. pp. 1–40.
  27. von Elbe JH, Schwartz SJ. Colorants. In: Fennema OR, editor. Food chemistry. 3rd ed. New York: Marcel Dekker; 1996. pp. 651–722.
  28. Lee J, Durst RW, Wrolstad RE. Determination of total monomeric anthocyanin pigment content of fruit juices, beverages, natural colorants, and wines by the pH differential method: Collaborative study. Journal of the AOAC International. 2005;88(5):1269–1278.
  29. Giusti MM, Wrolstad RE. Characterization and measurement of anthocyanins by UV–visible spectroscopy. In: Wrolstad RE, editor. Handbook of analytical food chemistry. New York: John Wiley; 2005. pp. 19–31.
  30. Türkyılmaz M. Anthocyanin and organic acid profiles of pomegranate (Punica granatum L.) juices from registered varieties in Turkey. International Journal of Food Science and Technology. 2013;48(10):2086–2095.
  31. Al-Abdulla By, Oleiwi SD. Estimation of some phytochemicals in extract of eggplant peels and cabbage leaves and using of pigment of them in drinks industry. Mesopotamia Journal of Agriculture. 2019;47(2):128–138.
  32. Rein M. Copigmentation reactions and color stability of berry anthocyanins. University of Helsinki, Department of Applied Chemistry and Microbiology, Food Chemistry Division, PhD Thesis; 2005.
  33. Starr MS, Francis FJ. Effect of metallic ions on color and pigment content of cranberry juice cocktail. Journal of Food Science. 1973;38(6):1043–1046.
  34. Markakis P. Stability of anthocyanins in foods. In: Markakis P, editor. Anthocyanins as food colors. New York: Academic Press; 1982. p. 163–178.
  35. Valavanidis A, Vlachogianni T. Plant polyphenols: Recent advances in epidemiological research and other studies on cancer prevention. Studies in Natural Products Chemistry. 2013;39:269–295.
  36. Osawa Y. Copigmentation of anthocyanins. In: Markakis P, editor. Anthocyanins as food colors. New York: Academic Press; 1982. pp. 41–65.
  37. Kulkarni SS, Gokhale AV, Bodake UM, Pathade GR. Cotton dyeing with natural dye extracted from pomegranate (Punica granatum) peel. Universal Journal of Environmental Research and Technology. 2011;1(2):135–139.
  38. Goodarzian H, Ekrami E. Wool dyeing with extracted dye from pomegranate (Punica granatum L.) peel. World Applied Sciences Journal. 2010;8(11):1387–1389.
  39. Jothi D. Extraction of natural dyes from African marigold flower (Tagates ereectal) for textile coloration. AUTEX Research Journal. 2008;8(2):49–53.
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