1887
Volume 2024, Issue 2
  • E-ISSN: 2223-506X

ملخص

The indigenous wild thyme () population in Libya exhibits a conspicuous and unique floral attribute, characterized by polymorphic flower coloration, which is discernible within a singular population. Thyme is classified as a Taxonomically Complex Group since it is a genetic combination of related individuals. Fifteen tender leaf samples were collected to capture regional floral diversity. Samples represented five flower colour categories (white, spotted-white, violet, pink, pied). Leaves were thoroughly washed with running distilled water for DNA extraction and sequencing. Sequences were then analysed and phylogenetic trees were constructed. Alignment of gene sequences from Libyan thyme samples exhibited that those with violet flowers displayed the highest similarity (96.67%) to the genus Thymbra capitata, whereas samples with white flowers demonstrated the lowest similarity (85%), while the sequences of white flowers displayed the highest similarity (99.63%), whereas the remaining tested samples exhibited a slightly lower similarity of 98%. Notably, the sequences of 14 thyme samples amplified using A primers have been deposited in the NCBI GenBank with accession numbers MW051762 to MW051775. In the context of analysis via and gene sequences using MEGA software, an unequivocal similarity was observed across the majority of examined thyme samples. DNA barcoding may not be the only key component required for species discrimination of developed in Libyan.

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References

  1. Anonymus. Vegetation cover, study and evaluation of vegetation cover in the Green Mountain region. The final report, Omar Al-Mukhtar University, 2005.
    [Google الباحث العلمي]
  2. Mabberley DJ. The plant-book: A portable dictionary of the vascular plants. Cambridge University Press. 1997; pp. 857. doi: https://doi.org/10.1017/9781316335581
    [Google الباحث العلمي]
  3. Džamić AM, Nikolić BJ, Giweli AA, Mitić-Ćulafić DS, Soković MD, Ristić MS, et al. Libyan Thymus capitatus essential oil: Antioxidant, antimicrobial, cytotoxic and colon pathogen adhesion-inhibition properties. Journal of Applied Microbiology. 2015; 119:(2):389–399. doi: https://doi.org/10.1111/jam.12864
    [Google الباحث العلمي]
  4. Bouyahya A, Chamkhi I, Guaouguaou F-E, Benali T, Balahbib A, El Omari N, et al. Ethnomedicinal use, phytochemistry, pharmacology, and food benefits of Thymus capitatus. Journal of Ethnopharmacology. 2020;259:112925. doi: https://doi.org/10.1016/j.jep.2020.112925
    [Google الباحث العلمي]
  5. Ali ES, Mustafa HM. Polymorphism in Thyme (Thymus capitatus) at southern region of El-Jabal El-Akhdar, Libya. Albayan Scientific Journal. 2019;4:47–62. https://search.mandumah.com/Record/1041388/Description
    [Google الباحث العلمي]
  6. Vaidya P, McDurmon A, Mattoon E, Keefe M, Carley L, Lee C-R et al. Ecological causes and consequences of flower color polymorphism in a self-pollinating plant (Boechera stricta). New Phytologist. 2018; 218:(1):380–392. doi: https://doi.org/10.1111/nph.14998
    [Google الباحث العلمي]
  7. Jones DA, Crawford TJ. Variation in the colour of the keel petals in Lotus corniculatus L. Heredity. 1977;39:313–325. doi: https://doi.org/10.1038/hdy.1977.72
    [Google الباحث العلمي]
  8. Clegg MT, Durbin ML. Flower colour variation: A model for the experimental study of evolution. Proceedings of the National Academy of Sciences of the United States of America. 2000; 97:(13):7016–7023. doi: https://doi.org/10.1073/pnas.97.13.7016
    [Google الباحث العلمي]
  9. Barcaccia G, Lucchin M, Cassandro M. DNA barcoding as a molecular tool to track down mislabeling and food piracy. Diversity. 2015; 8:(4):2. doi: https://doi.org/10.3390/d8010002
    [Google الباحث العلمي]
  10. De Mattia F, Bruni I, Galimberti A, Cattaneo F, Casiraghi M, Labra M. A comparative study of different DNA barcoding markers for the identification of some members of Lamiaceae. Food Research International. 2011;44:693–702. doi: https://doi.org/10.1016/j.foodres.2010.12.032
    [Google الباحث العلمي]
  11. CBOL Plant Working Group. A DNA barcode for land plants. Proceedings of the National Academy of Sciences of the United States of America. 2009; 106:(31):12794–12797. doi: https://doi.org/10.1073/pnas.0905845106
    [Google الباحث العلمي]
  12. Porebski S, Bailey LG, Baum BR. Modification of a CTAB DNA extraction protocol for plants containing high polysaccharide and polyphenol components. Plant Molecular Biology Reporter. 1997;15:8–15. doi: https://doi.org/10.1007/BF02772108
    [Google الباحث العلمي]
  13. Gouy M, Guindon S, Gascuel O. SeaView version 4: A multiplatform graphical user interface for sequence alignment and phylogenetic tree building. Molecular Biology and Evolution. 2010; 27:(2):221–224. doi: https://doi.org/10.1093/molbev/msp259
    [Google الباحث العلمي]
  14. Federici S, Galimberti A, Bartolucci F, Bruni I, De Mattia F, Cortis P, et al. DNA barcoding to analyse taxonomically complex groups in plants: The case of Thymus (Lamiaceae). Botanical Journal of the Linnean Society. 2013; 171:(4):687–699. doi: https://doi.org/10.1111/boj.12034
    [Google الباحث العلمي]
  15. Udensi OU, Ita EE, Ikpeme EV, Ubi G, Emeagi LI. Sequence analysis of Maturase K (matK): A chloroplast-encoding gene in some selected pulses. Global Journal of Pure and Applied Sciences. 2017; 23:(2):213–230. doi: https://doi.org/10.4314/gjpas.v23i2.2
    [Google الباحث العلمي]
  16. Wattoo JI, Saleem MZ, Shahzad MS, Arif A, Hameed A, Saleem MA. DNA barcoding: Amplification and sequence analysis of rbcL and matK genome regions in three divergent plant species. Advancements in Life Sciences. 2016; 4:(1):3–7.
    [Google الباحث العلمي]
  17. Thompson JD, Higgins DG, Gibson TJ. CLUSTAL W: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Research. 1994; 22:(22):4673–4680. doi: https://doi.org/10.1093/nar/22.22.4673
    [Google الباحث العلمي]
  18. Ennos R, French GC, Hollingsworth P. Conserving taxonomic complexity. Trends in Ecology and Evolution. 2005; 20:(4):164–168. doi: https://doi.org/10.1016/j.tree.2005.01.012
    [Google الباحث العلمي]
  19. Muellner AN, Schaefer H, Lahaye R. Evaluation of candidate DNA barcoding loci for economically important timber species of the mahogany family (Meliaceae). Molecular Ecology Resources. 2011; 11:(3):450–460. doi: https://doi.org/10.1111/j.1755-0998.2011.02984.x
    [Google الباحث العلمي]
  20. Mahdavi S, Karimzadeh G. Karyological and nuclear DNA content variation in some Iranian endemic Thymus species (Lamiaceae). Journal of Agricultural Science and Technology. 2010;12:447–458.
    [Google الباحث العلمي]
  21. Kress WJ, Wurdack KJ, Zimmer EA, Weigt LA, Janzen DH. Use of DNA barcodes to identify flowering plants. Proceedings of the National Academy of Sciences of the United States of America. 2005; 102:(23), 8369–8374. doi: https://doi.org/10.1073/pnas.050312310
    [Google الباحث العلمي]
  22. Manning JC, Goldblatt P, Snijman DA. The color and scent of flowers: An overview of pollination strategies in plants. Annals of Botany. 2009; 103:(9):1485–1498.
    [Google الباحث العلمي]
  23. Borsch T, Hilu, KWQuandt D, Wilde V, Neinhuis C, Barthlott W. Non-coding plastid trnT-trnF sequences reveal a well-resolved phylogeny of basal angiosperms. Journal of Evolutionary Biology. 2003; 16:(4):558–576. doi: https://doi.org/10.1046/j.1420-9101.2003.00577.x
    [Google الباحث العلمي]
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  • نوع المستند: Research Article
الموضوعات الرئيسية Thymus capitatus, floral polymorphism, DNA barcoding, matK and rbcL
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يُرجى إدخال عنوان بريد إلكتروني صالح
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