oa An Alternative Mechanism for Melatonin’s Protective Effects on Metal Ion Toxicity – Modulation of pro-/anti-apoptotic Proteins

Our objective was: Does Melatonin protect against cisplatin (Pt(II)) toxicity by a mechanism that is not free radical based? We used, flow cytometry, cell-viability, immunoblotting, and immunofluorescence, to determine the following: Cisplatin induced apoptosis is reversed by melatonin at physiological concentrations by localization of Bcl-2 and down regulation of Bax, besides reduction of oxidative stress.

Vignesh Shanmugam*, Amro Wafi, Elizabeth Varghese and Dietrich Büsselberg, Weill Cornell Medical College in Qatar