oa Dynamics of Myocardial Perfusion in Patients with Coronary Heart Disease and Post-infarction Cardiosclerosis After Stem Cell Therapy

Objective: To study efficacy and safety of autologous bone marrow stem cell therapy for tissue and neoangiogenesis in ischemic parts of heart in patients with coronary heart disease (CHD) and post-infarction cardiosclerosis after macro focal myocardial infarction.

Methods: Thirty patients with a diagnosis of ischemic heart diseases and myocardial infarction were selected. Fifteen of them were treated with the standard protocol of treatment (control group). The other 15 were transplanted with their bone marrow stem cells. Stem cells CD133 were isolated from mononuclear cells using Ficoll followed by immuno-magnetic separation. Isolated cells were injected in intra-arterial into the coronary arteries under angiography in the average dose of 5ml of suspension containing 0.8-1.5 million cells. Clinical examination and current tests such as ECG, LVEF, ESV, EDV were performed. In order to evaluate the dynamics of myocardial perfusion we carried out myocardial scintigraphy using Tc99m with the ligand methoxyisobutylisonitrile (MIBI).

Results: Key indicators of hemodynamic of heart were recorded before and after six months of the introduction of autologous stem cells. As presented in the table, the ejection fraction of left ventricle and end-diastolic volume of the left ventricle had a clear tendency to normalization.

Conclusions: Our method of stem cell transplantation is safe and does not increase mortality as a consequence of heart disease. Treatment of autologous stem cells significantly improved key indicators of heart hemodynamic. We thank J Al Majid from Dubai and H Sadeghi from Cardiovascular Surgery, University of Lausanne, for their precious help.

Z Rahimov1, A Barotov1, A Dustov2, M Khidirov3, T Gulmuradov1, G Mirojov2, B Karimzade2, N Navjuvonov 1, N Olimov1, E Rizoev1, A Gaibov2, M Rahmatov1, S Muminjonov2, A Didary3, J Irgashev3, A Therwath4,O Bobokhojaev1, U Kurbonov3, S Rahmonov1, M Mirshahi3,4.

1 Deptartment of Cardiology, Cardiovascular Surgery and Hematology of Ministry of Health of the Republic of Tajikistan, 2Institute of Gastroenterology of Tajikistan Academy of Medical Science, Dushanbe, Tajikistan, 3Deptartment of Surgical Diseases and Stem Cell Laboratory, Avicenna Tajik State Medical University, 4 UMRS 872, INSERM, Paris VI Faculty of Medicine, Paris, France