oa Propagation and Osteogenic Differentiation of Human Mesenchymal Stem Cells derived from Prenatal and Postnatal Sources

The application of mesenchymal stem cells differentiated into osteogenic cells is an attractive care modality to enhance the healing process of orthopedics and spinal injuries. Objectives of the in-vitro part of our study are: 1. To isolate and propagate Mesenchymal stem cells ( MSCs) derived from different sources; Bone Marrow (BM), Dental Pulp (DP), and Amniotic Fluid (AF) and 2. Induce their osteogenic differentiation in vitro.

Methods used were the generation and optimization of in-vitro cultures for DP- BM- and AF-MSCs, followed by their osteogenic differentiation by applying three independent induction protocols; osteogenic reagents containing media, bone morphologic protein2 (BMP2), and combined BMP2 and insulin growth factor (IGF).

The results showed that MSCs derived from different sources showed a significant difference both in the proliferation rate and osteogenic differentiation potentials. Whereas, DP-MSCs showed the highest proliferation rate, the AF-MSCs showed the greatest potential to osteogenic differentiation, whereas, BM-MSCs have the least potential toward osteogenic differentiation. In conclusion, the tissue of origin of derived MSCS is greatly influenced by the lineage of differentiation of derived stem cells.