Qatar Foundation Annual Research Forum Volume 2013 Issue 1

Synthesis and pharmacological screening of novel piperine derivatives as potential therapeutic agents to treat chronic kidney disease

Introduction: E. coli is usually harmless to the host, sometimes causes diarrhoeal and other diseases in humans. In the meantime, S. aureus is the cause of a wide range of different kinds of major and minor pyogenic infections. Rational : The mechanical action of the bacteriophage on the selected bacteria species depend on their receptors that adsorb them to their hosts, some proteins of the bacterial outer membrane acts as the receptor for phage infection, to isolate the phages and identify their species through electron microscopy analysis and/or DNA hybridization assays, the relationship between bacteriophages and bacteria proteins could be found by the protein profiles analysis Methods: The proteins of the Escherichia .coli phage and the E. coli bacteria were separated by Sodium Dodecyl Sulphate Poly Acrylamide Gel Electrophoresis (SDS-PAGE) to estimate their molecular weights; the migration of each band was compared to the standards of known weights in the molecular ladder. Results: The mobilised proteins of the phage showed three major bands with molecular weights of 46, 35 and 24 kDa. Meanwhile, the bacteria showed clear nine bands with molecular weight ranged between 96 and 14 kDa. This was analysed by the programme ImageJ136b which showed molecular masses of 47, 34 and 16 kDa, The analysed data showed the protein bands distance migration and plot area of the phage and the bacteria, the results in size from 47.7 to 34.3 kDa resembles 43.3% of total phage protein which formed the capsid head and the coil, while the molecular weight mass of 22.5 formed the tail proteins, we found that the band of 34 kDa was the common shared peak between them. Conclusion The bacterial host E. coli protein profile showed more bands and more peaks than the bacteriophages ones, this is related to the lytic cycle of the bacteriophages and their phenomenon in utilizing the bacterial DNA in proteins manufacturing during their multiplication inside the host.

Real-Time GPU Implementation of Correlation-Based Dense Matching of Stereo Endoscopic Images for Surface Reconstruction

The Polyamine Binding Protein, PotD, Is Needed for Intracellular Establishment of the Waterborne Pathogen Legionella pneumophila

Notch-Mediated Crosstalk between Breast Cancer Cells and Endothelial Cells Induces a Transitional EndMT Phenotype Participating to an Endothelial Pro-tumoral Niche

Cross-sectional study of total and central body fatness and c-reactive protein among Qatari men and women: The Qatar Biobank Project

Running mechanics and spring-mass behaviour during treadmill repeated sprints are different in hypoxia and in normoxia

Characterization of growth factor receptor signaling upon loss of endoplasmic reticulum chaperones

Standardization of an in vitro model of diabetic nephropathy using Madin-Darby Canine Kidney cells